Fig. 1

Generation of Dusp4-null progenitor-like primary mammary epithelial cells. A Schema for generation of a floxed murine Dusp4 allele. loxP sites flanking exons 3–4 were inserted using homology-directed recombination. The FRT-flanked neo cassette in the 3’ homology arm was excised following crossing to Flp deleter mice. B Bright-field images of Dusp4FLOX cells at 40X (scale bar = 50 µm), low and high confluency. C Dusp4 mRNA expression in Dusp4FLOX cells after transient infection with Ad.GFP or Ad.Cre