Fig. 3

Virgin and pregnant bcl-3^−/− mammary glands show increased apoptosis and undergo accelerated involution. a Mammary gland sections were stained for apoptotic cells using Apoptag in virgin mice and at the indicated days of pregnancy, lactation and involution. b The percentage of apoptotic epithelial cells were enumerated from a minimum of 3 glands. Statistical analysis; one-way ANOVA. c The percentage of adipose tissue was determined in mammary gland sections at 72hI from 3 dams per genotype. Statistical analysis; unpaired t test. A minimum of 500 cells were enumerated per gland. d Pups were removed at d10L and mammary glands processed for whole mount at 24hI and H&E sections at 24hI and 48hI. Image magnification is shown above. e Mean numbers of dead cells in alveoli at 48 h post-forced weaning were determined from at least 50 alveoli per gland (n = 3). Statistical analysis; unpaired t test. All bars are S.E.M