Fig. 4

Preventing weight gain after OVX influences the tumors and microenvironment. A Cell size distribution of subcutaneous/mammary adipocytes in lean and obese AdLib or WM rats at the end of study. B Mean adipocyte diameter of cells in subcutaneous/mammary depots at the end of study. Two-way ANOVA, main effects of adiposity, WM, or interaction. C Crown-like structure (CLS) scores, indicating local inflammation, in subcutaneous/mammary depots measured visually in blinded histological sections. D Representative images of IHC staining for CD68 + CLS. E Expression of Fgf1 in subcutaneous/mammary adipose tissue from lean or obese rats experiencing either a low or high rate of post-OVX weight gain and positive energy balance. Two-way ANOVA, main effects of adiposity or energy balance. F Expression of Fgf1 in subcutaneous/mammary adipose tissue from lean and obese AdLib or WM rats at the end of study, measured by qPCR. Two-way ANOVA, main effects of adiposity or WM. G Levels of phosphorylated FGFR1 (pFGFR1) in the tumors of lean and obese AdLib or WM rats evaluated by IHC and measured visually in blinded histological sections. Two-way ANOVA with Tukey’s post-hoc test. H Representative images of IHC staining for pFGFR in obese AdLib and WM tumors