Fig. 6
From: PTHrP induces STAT5 activation, secretory differentiation and accelerates mammary tumor development
PTHrP overexpression causes secretory differentiation and Stat5 activation in PyMT tumors. A H&E and immunohistochemical analysis of tumors from Tet-PTHrP;PyMT mice on Dox and controls. Representative images from each group are shown. N = 3. B Western Blots on protein lysates from whole tumors. Left shows Samples from three different mice per group were run with β-Actin as the loading control. Right shows the densitometric quantification of western blots. C QPCR analysis for the indicated genes of RNA isolated from whole tumors. Hprt1 was used as housekeeping gene. N = 3. D Left shows the custom GSEA for STAT5-dependent mammary gland genes comparing Tet-PTHrP;PyMT vs PyMT mice on dox. Nom p-value, normalized p value; FDR, false discovery rate; NES, normalized enrichment score. Right, heatmap depicting relative expression change of representative STAT5-dependent genes. Bars represent mean ± SEM, ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05