Fig. 1
Activation of NFκB by different ETs in preclinical ER + breast cancer models. a–c Clonogenic assays were conducted in MCF-7-NFκB-RE-GFP and T47D-NFκB-RE-GFP cell lines cultured in growth media (GM) in the presence or absence of 1 μM 4OHT or ICI, or in ED conditions for 2 weeks. a Colony confluence (area covered by colonies) was quantified using a Celigo imaging cytometer. b The percentage of GFP + confluence per condition was determined using Celigo imaging. c Representative images of colonies for the MCF-7-NFκB-RE-GFP cell line grown in GM ± 4OHT for 2 weeks. Scale bar: 200 µm. d HCI-003 and HCI-017 PDxOs were grown under the selective pressure of ETs for 2 weeks. Data represent total organoid area per µm2 on day 14 as determined by the Incucyte S3 organoid module. e, f Expression of ER target genes e and NFκB target genes f was determined by QPCR in HCI-003 and HCI-017 PDxOs treated with ET for 2 weeks. The ER target genes were used as controls for ET. The heatmap represents Fold Change for each gene relative to GM control