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Fig. 5 | Breast Cancer Research

Fig. 5

From: Breast cancer metastasis to brain results in recruitment and activation of microglia through annexin-A1/formyl peptide receptor signaling

Fig. 5

Extracellular and intracellular ANXA1 elicited different effects on migratory profiles in BV-2 microglial cells. a Primary adult microglia treated with either SFM, 4T07 CM, or 4T1 CM for 24 h and stained with ANXA1 (green), microglial marker Iba1 (red), and DNA-binding dye DAPI (blue). Graph (rightmost) showing the immunoreactivity against ANXA1 on primary adult microglial cells. b and c BV-2 or BV-2 ΔANXA1 microglia were treated with SFM, 4T1 CM or 4T1 ΔANXA1 CM for 24 h in the transwell migration assay. Representative brightfield images and quantification of migrated BV-2 or BV-2 ΔANXA1 microglia. c BV-2 or BV-2 ΔANXA1 microglia were treated with SFM, 4T1 CM or 4T1 ΔANXA1 CM for 24 h and gene expression of inflammatory markers were analyzed using qRT-PCR. Data represent mean ± SEM; n = 3 independent experiments. *P < 0.05, **P < 0.01

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Breast Cancer Research

ISSN: 1465-542X

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