Fig. 1

LncRNA IPW is downregulated in DCIS. A Schematic diagram of lncRNA profiling from DCIS and adjacent normal tissues. DCIS and adjacent normal tissues (n = 8 each) were homogenized and total RNAs were extracted from these samples. Expression of LncRNAs in SBI lncRNA profiler array was examined by qRT-PCR. B LncRNAs that were differentially expressed (fold change + 2) with statistical significance (p-value < 0.05) are listed in the table. C IPW expression was examined in 10A and DCIS (left panel), S1 and S2 (middle panel) and HMEC and SUM225 (right panel) by qRT-PCR (n = 5/group). Unpaired two-tailed Student’s t test was performed for statistical comparison. D IPW expression was examined by qRT-PCR in RNA extracted from normal, DCIS and IDC formalin-fixed paraffin-embedded slides by microdissection (n = 10/group). One-way ANOVA with Tukey multiple comparison post hoc test was performed. E IPW expression was examined in MCF10A (control) and MCF10A-shIPW cells (n = 5/group) by qRT PCR. Unpaired two-tailed Student’s t test was performed. F 3D culture was performed in Matrigel-coated 96 well plate. MCF10A, MCF10A-shIPW and DCIS.com cells were reconstituted in 10% Matrigel and seeded into Matrigel-coated plate. Representative bright-field images were acquired by microscopy at days 10–13 (n = 4/group). Scale bar 50 µm. G Acini formed in Figure F that were > 50uM in diameter were counted at days 10–13. Data are represented as mean + S.E.M (n = 4/group). (*p < 0.05, **p < 0.01, ****p < 0.0001). ns: not significant