Fig. 3

circCCDC85A acts as a miR-550a-5p sponge in breast cancer cells. A The potential miRNA targets of circCCDC85A were predicted by CircNet online database, and it was found that circCCDC85A may function as the sponge of miR-660-3p, miR-550a-5p and miR-4508. B The potential downstream pathways of the above three miRNAs were analyzed through tarbase database, and miR-550a-5p was found to be associated with the Hippo signaling pathway which is a well-known cancer-related pathway C FISH results showed that both circCCDC85A and miR-550a-5p were predominantly located in cytoplasm. D The miranda database was applied for prediction of the binding sites between circCCDC85A and miR-550a-5p, and it was found that there are two potential binding sites between hsa-miR-550a-5p and hsa_circ_0120472. E Immunoprecipitation of Myc-tagged AGO2 from MCF-7 cells transfected with either Myc-AGO2 or Myc-vector negative control plus miR-550a-5p mimics. The enrichment of circCCDC85A was detected by qRT-PCR. F Luciferase reporter assay was performed to detect the luciferase activity of luc-circCCDC85A or luc-vector negative control in MCF-7 cells co-transfected with miR-550a-5p mimics. *P < 0.05