Fig. 6

Triptonide inhibits tumorigenic Notch1 and NF-κB signaling pathways in TNBC cells. MDA-MB-231 and MDA-MB-468 cells were treated with triptonide (TN) for 72 h at the concentrations of 0–10 nM. Notch1 protein levels were detected by Western blotting (A, C), and the bands were scanned and statistically analyzed (B, D). Similarly, TN-mediated reduction of Notch1 protein levels in MDA-MB-468 cells was also verified (C, D). Additionally, the levels of total NF-κB protein and phosphorylated NF-κB protein (p-NF-κB) were also measured by Western blotting (E, G), and the bands were scanned and statistically analyzed (F, H). The mRNA levels of VE-cadherin, VEGFR2, and N-cadherin in MDA-MB-231 (I) and MDA-MB-468 (J) cells were assessed by real time quantitative PCR (QT-PCR). The results represent three independent experiments. *P < 0.05, **P < 0.01