Fig. 4

Modulation of Postn expression by FGFR and TGFβ signaling. A NeuNDL cells were treated in the presence of MEGS with or without 10 ng/ml of TGFβ-1 for 0.5 and 24 h and assessed for Postn protein levels. pSMAD2 (S465/467) was used as a control for the activation of the TGFβR. B Postn protein levels monitored in NeuNDL cells were treated with 5 µg/ml TGFβR inhibitor SB431542 or DMSO in either MEGS-depleted (PBS) or MEGS-supplemented medium for 0.5 and 24 h. pSMAD2 (S465/467) was used as a control for TFGβR inhibition. C MEGS-depleted (PBS) and BPE-supplemented media were pretreated with bFGF neutralizing antibody (nAb) or control IgG for 1 h at 4 °C prior to addition to the NeuNDL cultures and western blotting for Postn. D Immunofluorescence for SMAD2 nuclear localization was performed following a 24-h treatment with PBS, 10 ng/ml bFGF or 10 ng/ml TGFβ-1 of MEGS-depleted cultures. Manual counting of representative images was conducted to assess localization. Data are represented as mean ± SEM. N = 10, ***P ≤ 0.001 (D)