Fig. 2

Mammary epithelial growth supplement represses Postn expression in NeuNDL cells. A NeuNDL cells were grown in the presence or absence of FBS and MEGS as well as with neither and a combination of both for 24 h. Postn expression was assessed by western blotting analysis with β-actin as a loading control. B NeuNDL cells were seeded on coverslips, grown in the presence or absence of MEGS for 48 h and immunostained. Individual coverslips were stained for DAPI and POSTN. Scale bar = 100 µm C Representative images were counted to assess the percentage of POSTN expressing cells in both conditions. Data are represented as mean ± SEM. N = 10, ***P ≤ 0.001. D NeuNDL cells were treated with or without MEGS in varying FBS concentration from 0 to 10%, and Postn mRNA expression was assessed 24 h post-treatment. Data are represented as mean ± SEM. N = 3, ***P ≤ 0.001