Skip to main content

Table 1 List of antibodies, fluorophore conjugated to each, and the staining sequence in MxIF. Na+K+ATPase and Ribosomal S6 were used as segmentation markers for membrane and cytosol, respectively. Pan-cytokeratin (PCK26) was used as the epithelial cell marker. COX2 was also included in the staining rounds but data not analyzed in the current study

From: Quantitative single-cell analysis of immunofluorescence protein multiplex images illustrates biomarker spatial heterogeneity within breast cancer subtypes

Staining round

Cy3

Cy5

Antibody (clone)

Supplier

Cat #

Unconj or Conj

Antibody

Supplier

Cat #

Unconj or Conj

1

HER2/neu (SP3)

ThermoFisher

RM9103

Unconj

p16 (CINtec)

Roche/Ventana

725–413

Unconj

2

Na+K+ATPase

Abcam

ab167390

Conj

KI67(SP6)

Zeta

Z2031

Conj

3

PR

Dako

M3568

Conj

ER(SP1)

Spring Bioscience

M3014C

Conj

4

Ribosomal S6

Cell Signaling

2217BF

Conj

p21

Cell Signaling

2947BF

Conj

5

PCK26

Sigma

C5992

Conj

p53

Dako

M7001

Conj

6

–

   

COX2

ThermoFisher

187379

Conj

7

–

   

CD20

Abcam

ab166865

Conj

8

–

   

CD8

Dako

M7103

Conj