Fig. 5

Spatial analysis of EPH groups. A–C The centroid location of each single cell is marked in color according to their EPH group assignment. The corresponding virtual H&E image is shown in inset. Two Luminal B-like cases (HER2-) and a Luminal B-like (HER2+) case are shown. D–F Quantitative evaluation of spatial heterogeneity. With neighborhood analysis, the percentages of neighboring EPH groups for each central EPH group are calculated for the indicated distance (30 or 100 mm in radius). The Shannon Equitability index for each central cell type is presented as the mean and standard deviation. G–H Cumulative analysis of the proportion of each EPH cell type in the neighborhood of the central cell type for G Luminal A-like cancers (N = 5) and for H Luminal B-like (HER2-negative) cancers (N = 5)