Fig. 2

EMT-inducers were consistently upregulated, and EMT-repressors were downregulated after exposing breast cancer cells to fluid flow, as shown by heat maps of static and flow-exposed breast cancer cells (a, c, e, g). Microarray expression data for each cell line was validated with qPCR (b, d, f, h). Three independent replicates were run for each condition and relative gene expression was calculated using the comparative cycle threshold method. Red dotted lines indicate limits of fold change for differentially expressed genes