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Fig. 1 | Breast Cancer Research

Fig. 1

From: Oestrogen deprivation induces chemokine production and immune cell recruitment in in vitro and in vivo models of oestrogen receptor-positive breast cancer

Fig. 1

Oestrogen deprivation upregulates expression of chemokines a CCL5, b CXCL16 and c CCL22 in MCF-7 cells. MCF-7 cells were cultured with (+E2) or without (− E2) 1 × 10−9 M oestradiol, and mRNA expression of three chemokines was determined by RT-qPCR at 0, 24, 72 and 120 h. Data are normalised to the mean of three reference genes, FKBP15, TBP and PUM1. Error bars are SEM based on nine biological replicates performed across three independent experiments. P-values were calculated using unpaired t-tests

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Breast Cancer Research

ISSN: 1465-542X

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