Fig. 2

Sox9 binds directly to the SoxE binding sites within the Sox10 promoter. a The expression level of the SoxE family of transcription factors was assessed in SLK^fl/fl and SLK^-/- NDL cells by qRT-PCR analysis. b The levels of endogenous Sox10 were assessed in the indicated cell lines using qRT-PCR primers located within the 3′UTR of Sox10. Note that the retroviral pBABE-Sox10 plasmid only contains the coding cDNA and not the 3′UTR. c Chromatin immunoprecipitation (ChIP) was performed on SLK^fl/fl and SLK^-/- NDL cells to assess Sox9 binding to the Sox10 promoter. Following Sox9 ChIP, qPCR analysis was performed across two putative SoxE binding sites within the −6904/−5995 fragment of the Sox10 promoter. qRT-PCR data was normalized to an IgG ChIP (dashed red line) or a negative control element within exon one (−150/+103). N=3, nd: no detectable expression, ns: no statistical difference, ***p < 0.0005