Fig. 1
Identification of a differentially regulated fragment of the Sox10 promoter following Slk deletion. a SLK-null tumor cells do not differentially express known transcription factors that are AKT targets. Cytosolic (cyto) and nuclear (nuc) fractions were subjected to Western blot analysis for the indicated proteins. No changes were observed in levels or distribution of the AKT targets surveyed. b Schematic representation of putative SoxE binding sites within the Sox10 promoter. Luciferase assays from two multiple-species conserved enhancer sequences (MCS4 and 7) that have been shown to control Sox10 expression (c) and approximately 1 kb fragments of the Sox10 promoter (d) assessed for their ability to drive luciferase activity in SLKfl/fl and SLK-/- NDL cells. Raw light units from the luciferase constructs were normalized to Renilla for each technical sample. Data is represented as the mean luciferase activity from three independent biological replicates +/− SEM. ns: no statistical difference, *p < 0.05, ***p < 0.0005