Fig. 4

The effect of attenuated TGFB signalling on macrophage abundance and location in the mammary gland. Representative F4/80 antibody staining of macrophages around the ductal (a–c) and alveolar (d–f) epithelium. Examples of F4/80-positive epithelial cell-associated macrophages are indicated by arrows, and examples of F4/80-positive stromal macrophages are indicated by arrowheads. The number of F4/80-positive cells within the ductal epithelium and stroma (g) and within the alveolar epithelium and stroma (h) was calculated in the mammary glands from doxycycline-treated single transgenic control Cfms-rtTA (n = 10; a, d) mice, TetO-TbrII (n = 8; b, e) mice and double transgenic Cfms-TbrII (n = 12; c, f) mice. Data are presented as mean + SEM with statistical analysis using a one-way ANOVA test, *p < 0.05, **p < 0.01