Fig. 6

Multi-modal imaging of native cell death in the MCF10A_DCIS model of human DCIS. Two lesions were implanted in the lower mammary glands of three mice. Time series of axial optoacoustic images of C2Am and iC2Am, at the level of the lesion (a, top), and kidneys (a, bottom) in a representative animal. Time courses of optoacoustic (MSOT) signal in the two lesions are shown (b). Fluorescence imaging (FLI) ex vivo (c, top) of histological sections of the lesions shown in a. Histological sections (c, bottom) of lesions in a stained for CC3 and TUNEL; areas of staining (orange/brown) are highlighted against background signal (blue); level of cell death in the lesion is shown (lower right, %). Pearson correlation analyses (d) of C2Am and iC2Am optoacoustic signals with CC3 and TUNEL staining, of C2Am and iC2Am optoacoustic signals with the corresponding fluorescence signals, and of TUNEL staining with CC3 staining. Open circles (C2Am); closed circles (iC2Am). n = 3 mice, 6 lesions. Scale bars (a, b) = 10 mm, (c, d) = 4 mm. Dashed lines (a, top) indicate lesion location