Fig. 7
From: p66ShcA functions as a contextual promoter of breast cancer metastasis
Non-mitochondrial role for p66ShcA in increasing the growth of macroscopic breast tumor lung metastases. a Percentage of Ki67-positive cells, b cleaved Caspase-3 positive cells, and c 4HNE positive pixels in individual lung-metastatic lesions derived from 4T1-537 Parental, p66-CR (VC), p66-CR (WT), and p66-CR (S36A) breast cancer cells, both following tumor resection and following tail vein injection. Panel a: Parental, n = 109 lesions; p66-CR (VC), n = 90 lesions; p66-CR (WT), n = 116 lesions; p66-CR (S36A), n = 45 lesions. Panel b: Parental, n = 108 lesions; p66-CR (VC), n = 89 lesions; p66-CR (WT), n = 89 lesions; p66-CR (S36A), n = 45 lesions. Panel c: Parental, n = 111 lesions; p66-CR (VC), n = 95 lesions; p66-CR (WT), n = 116 lesions; p66-CR (S36A), n = 55 lesions. Representative IHC images are also shown. For panels a and c, statistical analysis was performed using a one-way ANOVA with a Tukey’s multiple comparisons test (*P < 0.05; ***P < 0.001; ****P < 0.0001)