Fig. 4

ESR1-depleted recurrences. a TMM-normalized expression of patient-matched local recurrences; primary tumor expression in blue, recurrent tumor expression in red. b Heatmap of differentially expressed genes (nominal p value < 0.05, red = high relative expression, blue = low relative expression) in ESR1-depleted recurrences versus matched primary tumors. Genes are sorted by p value and segregated by log2 fold-change values; log2 fold change > 0 on top, log2 fold change < 0 on bottom. c Ladder plots showing log2normCPM expression values for both KLK7 and PROM1, two of the most significantly upregulated genes in local recurrences with the largest average log2 fold changes. d ESR1 expression in metastatic, endocrine-resistant luminal tumors—MET500 (n = 47) and WCRC (n = 89) cohorts