Fig. 5

Correlations between PD-L1 expression in tumor samples by IHC and various immune parameters in peripheral blood by flow cytometry. Flow cytometry data from the eight patients that provided blood samples within 1 month of tumor biopsies (Fig. 4b) were correlated with PD-L1 scores in their tumors. Scores for PD-L1 staining from tumor biopsies in individual patients are shown on the x-axis in each panel with y-axes showing values for significantly correlated flow cytometry parameters from the same patients: a Granzyme B GMFI in CD56^dim NK cells, b granzyme B GMFI in CD4⁺ T cells, c perforin GMFI in CD4⁺ T cells, d numbers of CD8⁺ effector memory T cells/μl of peripheral blood, e % of total CD8⁺ T cells with the naïve phenotype. Datapoints represent the maximum measured PD-L1 values in cases where a second measurement was made, as in Fig. 4. P and R values were computed from a Spearman test. The lines are least-squares fits to the data that are provided for visual purposes