Fig. 2
EVs isolated from YWBC patients promote increased invasion of MCF10DCIS.com breast cancer cells. EVs isolated from human YWBC patients or healthy donor (HD) plasma samples by size-exclusion chromatography were incubated with MCF10DCIS.com human breast cancer cells in a scratch wound assay overlaid with a Matrigel pad. Phase images were taken every 2 h using an IncuCyte instrument. After 48 h, images were analyzed using IncuCyte ZOOM software. a Mean diameter and number of EVs isolated from YWBC plasma or HD plasma. b Electron microscope images of YWBC EV and HD EV. c Representative images of the invasion assay showing the initial scratch wound (t0) and after 20 h (t20). The percentage invasion at each time point was calculated from the density of cells in each wound (shown in yellow) relative to the initial wound (shown in purple). d The average percent invasion of 4 replicate wells was plotted over time. Representative data for untreated MCF10DCIS.com cells (gray) or treated with EVs from a YWBC patient (blue) or HD (black). e The average percent invasion at the time point when untreated controls reached 50% confluence was compared to individual treatments using Student’s t test (left). The average percent invasion for each condition was compared using one-way ANOVA with a multiple comparisons test (right). There was no significant difference between untreated cells and those treated with EVs from healthy donors