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Fig. 1 | Breast Cancer Research

Fig. 1

From: Activating transcription factor-2 (ATF2) is a key determinant of resistance to endocrine treatment in an in vitro model of breast cancer

Fig. 1

Effect of knockdown of ATF2 on growth and tumourigenesis of MCF7 and TAMR (MCF7-derived tamoxifen-resistant) cell lines. MCF7 (a) and TAMR (b) cells were transfected with negative control siRNA (siControl), ATF2-siRNA1 and ATF2-siRNA2. Protein lysates were prepared and immunoblotting was carried out for ATF2 with β-actin as a loading control. c Densitometry analysis of knockdown efficiency relative to untransfected cells. SRB growth assay for MCF7 (d) and TAMR (e) indicated a significant growth reduction in TAMR cells after ATF2 silencing (n = 3). f Graph indicating the percentage of growth at day 5 relative to untransfected cells. g The effect of ATF2 knockdown on tumourigenesis was determined by anchorage-independent colony formation. Although there was a reduction of colonies in the MCF7 cells after ATF2 silencing, this reduction was more profound in the TAMRs (n = 3). Asterisks indicate statistically significant (*p < 0.05, **p < 0.005) difference from untransfected cells

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Breast Cancer Research

ISSN: 1465-542X

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