Fig. 5

Dormant tumor cell lines establish mammary carcinoma and distant tumor dormancy in FVBN202 transgenic mice. Female FVBN202 transgenic mice (8–10 weeks old) were challenged in the mammary region with the dormant tumor cell lines (R-Ctrl, R-FAC, R-FAC/AIT, 3 million cells/mouse) which were recovered from the lungs of FVBN202 transgenic mice bearing primary mammary carcinoma. a Tumor growth in the mammary region was measured using a digital caliper. Percent total neu+ dormant tumor cells in the lungs (b) or in the liver (e) were detected on FVS negative (FVS−) viable cells (%total neu+ cells). Ki67 expression is shown on FVS− neu+ cells. c–f Gated FVS−CD3+ T cells were analyzed for CD4+ or CD8+ T cells in the lungs (c, d) or in the liver (e, f). Gated FVS−CD3+CD4+ or CD8+ T cells were analyzed for T effector cells (Te, CD44+CD62L−), T effector/memory cells (Tem, CD44+CD62L^low), T central/memory cells (Tcm, CD44+CD62L^high), or T naïve cells (Tn, CD44−CD62L+) in the lungs (c, d) or in the liver (e, f)