Fig. 4

Sunitinib-induced senescent ECs exhibited increased VCAM1 expression and a greater TC/EC interaction. a The mRNA level of VCAM-1 in HUVECs treated with vehicle or sunitinib was measured by real-time RT-PCR. b HUVECs treated with vehicle or sunitinib were subjected to WB assay to test the VCAM-1 expression (the mean level in the control group relative to β-actin was set to 1.0; n = 3). c EGFP-transfected 4T1 or MDA-MB-231 cells were co-cultured with HUVEC monolayer that was pre-treated with vehicle or sunitinib (20 μM). The cells that did not adhere to the ECs were washed using PBS. d The number of 4T1 or MDA-MB-231 cells that adhered to HUVECs was quantified using 10 randomly chosen fields from two experiments. Data were presented as columns with mean ± SEM. Significance was assessed by unpaired t test with Welch’s correction