Fig. 6
From: Fibroblasts direct differentiation of human breast epithelial progenitors
HBFCCD105 TGF-β signaling supports parenchymal morphogenesis. a Overview of experimental design in which HBFCs are plated and exposed to 10 μM SB431542 or vehicle (DMSO) from day 7 to day 10 at which SB431542 or vehicle are removed and primary CD271low/MUC1high luminal breast epithelial cells are added and co-cultured for 10 days prior to assessment of epithelial structure formation. b 15 tests representing recombinations of four fibroblast biopsies and five epithelial biopsies are presented in a paired dot plot and a representative set of micrographs, showing a significant reduction in epithelial structure formation per square unit area in response to SB431542 versus vehicle in HBFCCD105 co-cultures only (asterisk indicates significance at p < 0.05 by Wilcoxon signed-rank test, ns = not significant) (bar = 1000 μm)