Fig. 3

ERα-36 regulates PR expression. a ERα-36 and actin mRNA expression were analyzed by RT-PCR in T47D clones: F4 (WT) and A6 (KO-ER36) and G3 (KO-ERα-36). b PR, ERα, and tubulin expression were assessed by Western blot in the three clones. Western blot quantification was determined comparing PR to tubulin using ChemiDoc MP (Biorad) to measure the chemiluminescence from the immunoblots. The values represent the mean ± SEM of three independent experiments. The p value was determined comparing each ERα-36 KO clones to the WT using Student’s t test. *p < 0.05, **p < 0.01. c PR expression was studied by immunofluorescence in the 3 clones. The nuclei were counterstained with DAPI (blue) (Obj, × 40). d Total RNA was prepared and cDNAs analyzed by RT-qPCR with specific primers for PR. The values were normalized against 28S mRNA and represent the mean ± SEM of three experiments. The p value was determined comparing each ERα-36 KO clone to WT cells using Student’s t test. **p < 0.01, **p < 0.001