Fig. 1

In utero BPA exposure drives long-term changes in mammary fibroblast gene expression. Fibroblasts were isolated from the mammary glands of 12-week-old female mice exposed in utero to 25 μg/kg · bodyweight BPA or oil control from E9.5 through E18.5. RNA from the fibroblasts was sequenced and analyzed for differential gene expression. a A volcano plot is depicted of all analyzed genes with cutoffs for fold change, p value, and p adjusted (padj) value depicted. b A heatmap of the 47 genes with a padj value ≤ 0.05 is depicted illustrating the fold change between BPA- and oil-exposed fibroblasts in each of the three cohorts of mice used for the transcriptome analysis. These analyses were done in 3 separate cohorts of mice treated with either oil or BPA in utero