Fig. 4

Loss of anillin attenuates primary breast tumor growth and inhibits tumor metastasis in vivo. Control and three different anillin knockout MDA-MB-231 cell lines stable-expressing a luciferase construct were injected into the mammary gland of NSG mice. a Tumor volume was measured starting at day 7 after injection of the cells, whereas weight and total luciferase intensity of dissected tumors was measured 6 weeks after the injection at the end of the experiment. b Luciferase intensity in isolated liver, lungs, kidney, ovary, and lymph nodes was measured by IVIS at the end of the experiment. Data is presented as mean ± SE (n = 10–11); *p < 0.05; **p < 0.01; ***p < 0.001