Fig. 3

Anillin is not essential for anchorage-dependent cell proliferation, but is critical for the anchorage-independent growth of breast cancer cells. Control and anillin-depleted MDA-MB-231 cells (a), as well as control and anillin-overexpressing MCF10AneoT cells (b) were cultured on plastic plates. The number of cells at different time point was counted using a hemocytometer (n = 3). Control and anillin-depleted MDA-MB-231 cells (c,d), as well as control and anillin-overexpressing MCF10AneoT cells (e,f) were cultured on soft agar. The number of cell colonies was counted on days 40 (MDA-MB-231) or 35 (MCF10AneoT) after plating. Data is presented as mean ± SE (n = 3); ***p < 0.001