Fig. 2

Increased anillin expression promotes collective migration and Matrigel invasion of breast cancer cells. Endogenous anillin expression was increased in MCF10AneoT cells using a specific CRISPR/Cas9-driving promoter-activation plasmid (anillin activator). a Immunoblotting analysis shows the magnitude of anillin overexpression. b,c Representative images and quantification of the collective migration of control and anillin-overexpressing MCF10AneoT cell monolayers at 8 h post-wounding. Scale bar, 100 μm. d,e Representative images and quantitative analysis of DAPI-labeled control and anillin-overexpressing cells after 24 h invasion into Matrigel. Data are presented as mean ± SE (n = 3); ***p < 0.001; ****p < 0.0001, as compared to the control group. Scale bar, 100 μm