Fig. 1

Loss of anillin expression inhibits collective migration and Matrigel invasion of breast cancer cells. Anillin expression was downregulated in MDA-MB-231 cells using CRISPR/Cas9-mediated gene editing. a Immunoblotting analysis shows the efficiency of anillin knockout by four different single-guide (sg) RNAs. b,c Representative images and quantification of the wound healing in control and anillin-depleted MDA-MB-231 cell monolayers at 8 h post-wounding. d,e Representative images and quantitative analysis of DAPI-labeled control and anillin-deficient MDA-MB-231 cells after 24 h invasion into Matrigel. Data are presented as mean ± SE (n = 3); **p < 0.01; ***p < 0.001, as compared to the control sgRNA-transfected group. Scale bars, 200 μm