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Fig. 2 | Breast Cancer Research

Fig. 2

From: Cross-talk between SIM2s and NFκB regulates cyclooxygenase 2 expression in breast cancer

Fig. 2

a SIM2s expression in DCIS.COM control cells and cells overexpressing IKKβ by qPCR as fold change. b SIM2s expression in DCIS.COM control cells and cells overexpressing IκB-SR by qPCR as fold change. c SIM2s promoter activity in MCF7 cells co-transfected with SIM2 promoter upstream of the luciferase gene and increasing amounts of NFκB p65 (50 ng, 100 ng, and 150 ng). d SIM2s promoter activity in MCF7 cells after co-transfection of promoter with control vector (pcDNA3), NFκB p65, and/or IκB-SR. e ChIP assay for NFκB p65 binding after transient transfection of SIM2 promoter with NFκB p65 in HEK293T cells. f SIM2s promoter activity in MCF7 cells co-transfected with SIM2s promoter upstream of the luciferase gene and 150 ng NFκB p65 compared with the SIM2s promoter activity in MCF7 cells co-transfected with NFκB double mutant SIM2s promoter upstream of the luciferase gene. ANOVA and Student’s t test were performed to test significance. a, b All significant at p < 0.05, unpaired t test: *p < 0.05

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