Fig. 4

Unsupervised hierarchical clustering (Pearson, ward.D2) of 902 genes whose expression was significantly regulated after 2 weeks of treatment in HER2− tumours. And the overrepresented pathways (FDR < 5%) identified by pathway analysis (IPA). a The relative change in the gene expression across 134 HER2− tumours was standardised (centred and scaled). Red denotes the standardised z-score > 0, an increase in gene expression in a tumour after AI treatment compared to the average “relative changes” of the gene across all the 134 tumours; blue denotes the standardised z-score < 0, a decrease in gene expression in a tumour after AI treatment compared to the average “relative changes” of the gene across all the 134 tumours. b The 25 canonical pathways were significantly enriched (FDR < 5%). Positive z-score shown in orange colour specifies activated pathways; negative z-score shown in blue colour specifies inhibited pathways after AI treatment