Fig. 1
From: BRCA1 mutation influences progesterone response in human benign mammary organoids
Generation of human mammary organoids. a Top, Schematic of overall study design is shown. Bottom panel is the 28-day stepwise menstrual cycle hormone profile. Day 0–day 14 Follicular phase, day 14–day 28 luteal phase. b PR and ER were co-stained with luminal marker (CK18) in BRCA1mut and non-carrier organoids. Immunofluorescent staining was done for ER (red) and PR (red) along with CK18 (green) and DAPI (blue) to visualize the nuclei. Scale bar, 100 μm. Co-localization is shown by red arrows. c Normalized expression of ER and PR mRNA in BRCA1mut and non-carrier breast organoids is shown. Unpaired t-test was performed