Fig. 4
Spy1 can enhance proliferation in the presence of p53. a HC11 cells were transfected with vector control, pEIZ-Spy1, p53 or pEIZ-Spy1 and p53 in the presence or absence of 1.5 μg/mL of DMBA. Levels of Spy1 are depicted (upper panels). Growth of cells following transfection was assessed via trypan blue analysis (lower panels) (n = 3). b MCF7 (left panel) and MDA-MB 231 (right panel) were transfected with vector control, pEIZ-Spy1, p53 or pEIZ-Spy1 and p53 in the presence or absence of 50 J/m2 UV damage. Growth of cells following transfection was assessed via trypan blue analysis (n = 3). c qRT-PCR analysis of p53 levels in littermate control (F1 Cntl) and MMTV-Spy1 primary mammary epithelial cells corrected for total GAPDH (left panel). Quantification of BrdU-positive cells with and without UV irradiation with (siCntl) and without p53 (sip53) (right panel). F1 Cntl n = 5, MMTV-Spy1 n = 5. Error bars represent SE; Student’s T test. *p < 0.05, **p < 0.01, ***p < 0.001