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Fig. 4 | Breast Cancer Research

Fig. 4

From: Loss of SIM2s inhibits RAD51 binding and leads to unresolved replication stress

Fig. 4

Loss of SIM2s leads to a decrease in RAD51 foci MCF7 cells. MCF7 cells containing shSIM2 or pSIL were treated with 0.5 mM HU, fixed 24 h later, and finally probed with the indicated antibody. Cells containing 10 or more foci were considered to be positive for the factors indicated as has previously been demonstrated [13]. Scale bars, 10 μm. Values indicate the mean ± SE with n ≥ 100 cells. Student’s t test was performed to test significance. *p value < 0.05

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