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Fig. 1 | Breast Cancer Research

Fig. 1

From: Loss of SIM2s inhibits RAD51 binding and leads to unresolved replication stress

Fig. 1

Loss of SIM2s leads to an increase in replication fork collapse. a Illustration of experimental design and raw measurements collected from DNA combing assays in MCF7-shSIM2 and MCF7-pSIL for experiments in b and c. b, c Visualization of IdU tract lengths from MCF7-shSIM2 and MCF7-pSIL cells treated with 10 mM HU or no treatment. d Illustration of experimental design and raw measurements for DNA combing assays for f and g. e Representative images enhanced for clarity of DNA tracts isolated from the indicated treatment group. f Visualization of IdU tract lengths from MCF7-shSIM2 and MCF7-pSIL cells treated with DMSO or 10 mM HU. g Visualization of CldU tract lengths from MCF7-shSIM2 and MCF7-pSIL cells treated with DMSO or 10 mM HU. Values indicate the median with the interquartile range. Whiskers span 5-95th percentile; n ≥ 100 strands. Student’s t test was performed to test significance. All scale bars, 1 μm. *p value < 0.05

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