Fig. 2

Expression patterns and proliferation rates of triple-negative breast cancer cells with altered CRYβB2 and CRYβB2P1. a, b Immunoblot and qRT-PCR analysis of the indicated cell lines with altered CRYβB2 and CRYβB2P1. Overexpression and knockout clonal populations were generated and selected via lentiviral transduction and CRISPR/Cas9-mediated transfection, flow cytometry, and antibiotic selection. c Immunofluorescence demonstrating the localization and expression of CRYβB2 in the SUM159 cell models was performed using an anti-CRYβB2 primary antibody and an anti-rabbit Alexa Fluor-488(green)-labeled secondary antibody. DNA; DAPI (blue), imaged at × 40. d Cells were plated at 25,000 cells per well in triplicate and counted every 24 h for 96 h. All data represent mean ± SE of a minimum of three independent experiments. *p < 0.01. 231, MDA-MD231; +C, CRYBB2 overexpression; +P1, CRYβB2P1 overexpression; P-/-, CRYβB2P1 knockout