Fig. 4
Effect of miR-671-5p on cell invasion. a Transwell Matrigel assays were performed for the invasion activity of 21T cell lines transfected with mock control, miR-671-5p mimic, inhibitor control, and miR-671-5p inhibitor. Overexpression of miR-671-5p significantly inhibits cell invasion in 21PT, 21NT, and 21MT cells, but slightly in H16N2 cells. b Transwell Matrigel assays showed that re-expression of FOXM1 abrogated invasion suppression by miR-671-5p in 21TP, 21NT and 21MT cells. Invasion ability of the cells was displayed as a percentage of the absolute cell numbers (right). Five fields of unit area on each membrane or whole membrane were counted for cell numbers, and the experiments were repeated three times with triplicates. Results are displayed as mean data ± S.D. (*p < 0.05)