Fig. 5
From: Vasculogenic mimicry is associated with trastuzumab resistance of HER2-positive breast cancer
Tzm-resistant HER2+ breast cancer cells exhibited VM. a Tube formation assay using three Tzm-resistant cell lines. Representative photographs are shown. Scale bar, 0.5 mm. b The numbers of tubes formed are shown (n = 4; mean ± SD). ns., not significant; **P < 0.01. c Western blots of various VM markers and angiogenic growth factor receptors in Tzm-resistant cells cultured overnight on Matrigel in the medium used for the tube formation assay. HUVECs served as positive controls for FGFR1 and VEGFR1. d The effect of each angiogenic growth factor (GF), including EGF, FGF2, IGF1, and VEGF, on tube formation. The numbers of tubes formed are shown (n = 4; mean ± SD). ns., not significant; *P < 0.05; **P < 0.01