Fig. 4

Expression of cytokeratin, NFYA, and YWHAB in CTCs isolated from breast cancer patients. a Quantification of NFYA expression (intensity per pixel) in breast cancer cell lines, Hela cells, PBMCs, and patients’ CTCs, using ARIOL system automate software. b Quantification of YWHAB expression (intensity per pixel) in breast cancer cell lines, in Hela cells, in PBMCs, and in patients’ CTCs, using ARIOL system automate software. c Cytospins obtained from metastatic breast cancer patients were triple stained with CK (green), YWHAB (orange), and NFYA (red) antibodies plus DAPI (blue) and analyzed with ARIOL system (magnification × 40). d Representative images from confocal laser scanning microscopy. Patients’ samples were triple stained with CK (green), NFYA (blue), and YWHAB (red) antibodies plus DAPI (not shown due to the absence of the corresponding laser) and analyzed with confocal laser scanning microscopy (magnification × 60)