Fig. 2

Expression of cytokeratin, CXCR4, and JUNB in CTCs isolated from breast cancer patients. a Quantification of CXCR4 expression (intensity per pixel) in breast cancer cell lines, Hela cells, PBMCs, and patients’ CTCs, using ARIOL system automate software. b Quantification of JUNB expression (intensity per pixel) in breast cancer cell lines, in Hela cells, PBMCs, and patients’ CTCs, using ARIOL system automate software. c Cytospins obtained from metastatic breast cancer patients were triple stained with CK (green), CXCR4 (orange), and JUNB (red) antibodies plus DAPI (blue) and analyzed with ARIOL system (magnification X40). d Representative images from confocal laser scanning microscopy. Patients’ samples were triple stained with CK (green), CXCR4 (red), and JUNB (blue) antibodies plus DAPI (not shown due to the absence of corresponding laser) magnification (× 60)