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Fig. 5 | Breast Cancer Research

Fig. 5

From: Glucocorticoid receptor modulation decreases ER-positive breast cancer cell proliferation and suppresses wild-type and mutant ER chromatin association

Fig. 5

SGRM-mediated GR chromatin association also results in reduced Y537S mutant ER chromatin association at the CCND1 enhancer region. MCF-7 HA-tagged Y537S cells were pre-treated for 48 h with 0.5 μg/mL doxycycline to induce the expression of HA-tagged Y537S ER and treated with vehicle (Veh, ETOH), 100 nM dexamethasone (Dex), 1 μM C134 (134), or 1 μM C335 (335) for 60 min. a GR ChIP analysis in HA-Y537S mutant cells revealed consistent GR occupancy at known ER enhancer regions (CCND1, CDK2, and CDK6) following either Dex or SGRM treatment. b HA-ER Y537S enrichment was suppressed with C335 and C134 at 60 min at the CCND1 and with C335 at the CDK2 enhancers (***p < 0.001, **p < 0.01, *p < 0.05 vs. Veh, one-way ANOVA, Dunnett’s post hoc test, n = 3 ±SD)

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