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Table 4 Inhibition of SIRT1 expression enhances the chemo-sensitivities to ADM and PTX in breast cancer cells overexpressing NNMT

From: Nicotinamide N-methyltransferase enhances chemoresistance in breast cancer through SIRT1 protein stabilization

  ADM (μM) PTX (nM)
Cell lines Mean ± SEM Fold change Mean ± SEM Fold change
SK-BR-3/NNMT-2 1.02 ± 0.09   25.63 ± 2.06  
SK-BR-3/NNMT-2+EX527 0.88 ± 0.08 0.86 21.10 ± 1.67 0.82
SK-BR-3/NNMT-2+SIRT1 siRNA 0.49 ± 0.06** 0.48 17.37 ± 1.56* 0.68
MCF7/NNMT-2 0.95 ± 0.08   31.92 ± 2.49  
MCF7/NNMT-2+EX527 0.53 ± 0.06* 0.56 25.15 ± 1.53 0.79
MCF7/NNMT-2+SIRT1 siRNA 0.35 ± 0.05** 0.37 21.95 ± 1.39* 0.69
MDA-MB-231 1.23 ± 0.09   12.40 ± 1.13  
MDA-MB-231+EX527 0.92 ± 0.11 0.75 7.20 ± 0.78* 0.58
MDA-MB-231+SIRT1 siRNA 0.64 ± 0.05** 0.52 6.10 ± 0.74** 0.49
  1. Data represent the mean ± SEM of three independent experiments. Statistical significance was detected between the EX527, SIRT1 siRNA groups, and control groups treated only with DMSO
  2. *p < 0.05, **p < 0.01