Fig. 4
From: Absence of integrin α3β1 promotes the progression of HER2-driven breast cancer in vivo
Decreased ITGA3 expression in HER2+, compared to triple-negative human carcinoma cells. a Flow cytometry histograms of signal intensity for α3 (left) and HER2 (right) staining of six human mammary carcinoma cell lines show lower surface expression of α3 in HER2+ cells. 50 × 103 cells were analyzed per experiment; representative graphs of three independent experiments. b Scatter plot of ITGA3 and ERBB2 expression for HER2+ and triple-negative-enriched breast cancer panel [22] shows a negative correlation between ITGA3 and ERBB2 expression (Spearman’s rho − 0.46, P = 0.01, n = 30). c Scatter plot of ITGA3 gene copy number estimates against ITGA3 expression for HER2+ and triple-negative-enriched breast cancer panel [22]. Despite ITGA3 amplification in several HER2+ cell lines, their expression of ITGA3 remains relatively low