Fig. 3

H-scores and representative images from IHC of untreated MAS98.06 and MAS98.12 tumors. The tumors were stained with antibodies against aldehyde dehydrogenase 18 family member A1 (ALDH18A1), cMYC, glutamate dehydrogenase 1 (GLUD1), glutaminase (GLS1), glutamine synthetase (GS), pyrroline-5-carboxylate reductase 1 (PYCR1), and solute carrier family 1 member 5 (SLC1A5). Left columns present the percentage areas with different intensity levels (DAB: 3′-diaminobenzidine) for MAS98.06 (blue shades), and MAS98.12 (red shades) tumors. Neg, no labeling; 1+, light label; 2+, moderate label; 3+, dark label. Right columns show representative IHC images for each antibody for both MAS98.06 tumors (left) and MAS98.12 tumors (right) in two different magnifications of × 4 and × 20. Scale bar is 200 μm for × 4 magnification and 50 μm for × 20 magnification. *p < 0.05, ***p < 0.001, *****p < 0.00001