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Fig. 4 | Breast Cancer Research

Fig. 4

From: BRCA1 mutations attenuate super-enhancer function and chromatin looping in haploinsufficient human breast epithelial cells

Fig. 4

Reduced H3K27ac levels in BRCA1185delAG/+ MCF10A clones are associated with attenuated enhancer function and transcription. a Relative BRCA1 binding at SOD2 and TNFAIP3 super-enhancers in WT MCF10A cells. IgG binding was used as the control. The graph is an average of three independent experiments. b Relative BRD4 binding at SOD2 and TNFAIP3 super-enhancers. A desert zone with no genes or enhancers within 100 kb was used as the negative control. The graph is an average of three independent experiments with WT MCF10A and BRCA1185delAG/+ MCF10A cells. c Relative MED1 binding at SOD2 and TNFAIP3 super-enhancers. A desert zone with no genes or enhancers within 100 kb was used as the control. The graph is an average of three independent experiments. d Relative CTCF binding at SOD2 super-enhancer. Two known CTCF peaks (left and right) located within SOD2 super-enhancer were investigated. A desert zone with no CTCF binding was used as the control. The graph is an average of three independent experiments. e Relative mRNA expression of TNFAIP3 and SOD2. The graph is an average of five independent experiments. *P < 0.05 by two-tailed t test. n.s. not significant by two-tailed t test. Error bars represent s.e.m

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