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Fig. 1 | Breast Cancer Research

Fig. 1

From: Targeting promiscuous heterodimerization overcomes innate resistance to ERBB2 dimerization inhibitors in breast cancer

Fig. 1

Targeting ERBB2 with therapeutic monoclonal antibodies. a Western blotting showing the expression of ERBB2, pAktS473 and Akt in a panel of breast cancer cell lines. b Cell viability assays performed on the panel of lines with trastuzumab and pertuzumab at the concentrations indicated for 5 days (n = 6, mean ± SD). c Combinatorial cell viability assays with trastuzumab and pertuzumab at the concentrations indicated for 5 days (n = 6, mean). Raw data is presented in Additional file 2: Figure S1B. d Western blotting showing the phosphorylation of ERBB3Y1289 and AktS473 following treatment with trastuzumab (100 nM), pertuzumab (100 nM) and the combination of both, at the time points indicated. Blots were re-probed with an actin antibody for quantification. A representative image is shown from three independent replicates (n = 3, mean ± SD). All drug treatments were significantly different from control. For simplicity, the indicated significance compares the combination to trastuzumab only (*p < 0.05, **p < 0.01)

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